Countercurrent chromatography, one of the oldest techniques for achieving
separation, is based on partitioning of solutes between a stream of liquid
mobile phase and a liquid stationary phase. EYLA countercurrent
chromatographs offers several
inherent advantages; large sample capacity, small solvent consumption, inert
environment and low operating pressure. In addition, simplicity of operation and the
ability to separate anything from milligram to gram quantities has led to the
widespread acceptance, especially in the field of natural products research.
Droplet Countercurrent Chromatograph
The mobile phase in the form of droplets is pumped into series of columns containing the immiscible stationary liquid phase. When the sample components are introduced into the mobile phase, separation is achieved due to the difference in the partition coefficient between the two phases. Several kinds of liquid are can be used for the two immiscible phases, such as water, methanol and chloroform. The DCC-3000 includes 300 columns of 400mm length enclosed in a thermostated oven. The column hardware can either be glass or Teflon.
Rotation Locular Countercurrent Chromatograph
Separation of solutes is achieved by partitioning between a steady stream of mobile phase and the stationary phase filled in the locular column system. The RLCC-3000 consists of 16 glass columns, of 500mm length, each containing 37 chambers or locules formed by Teflon disks and spacers. The RLCC-1000 can be used with wide range of solvent system, including emulsive solvent